A new antibiotic, gougeroxymycin.

The strain MA428-C1 was shaken-cultured (120 strokes/min., 8 cm amplitude) in. a medium (pH7.0) containing 2% glucose, 1.5% soybean meal, 0.3% NaCl, 0.1% K2HPO4, 0.1 % MgSO4-7H2O, 0.0007 % CuSO4-5H2O, 0.0001 % FeSO4-7H2O, 0.0008 % MnCl2-4H2O, 0.0002% ZnSO4-7H2O at 27°C for 5 days. The amount of gougeroxymycin was determined by a cylinder plate method described by one of the authors1}.. About 70 % of the gougeroxymycin in the cultured broth was in the mycelium mass; the rest was in the liquid. The antibiotic in the liquid was extracted with w-butanol,, and that in the mycelium cake with methanol. For example, 14 liters of cultured broth containing 14.6 mcg/ml of gougeroxymycin was filtered and the filtrate extracted, twice with 3.5 liters of w-butanol. The myceliumcake was extracted five times with 1 liter of methanol, and the methanol extracts were combined, cencentrated in vacuo,, and the concentrated solution extracted with n-butanol. The w-butanol extracts,